MOTS-C is a remarkable peptide meticulously designed for scientific inquiry. This product offers a high purity rate of 99%. It provides reliable results for various laboratory applications. MOTS-C is popular among researchers who require quality and precision in their studies.
MOTS-c features a 16-amino-acid sequence: MRWQEMGYIFYPRKLR, derived from a 51-base-pair short open reading frame (sORF) in the mitochondrial 12S rRNA gene (MT-RNR1), with high conservation across mammalian species, particularly in the first 11 residues. Its molecular weight is approximately 2,019 Da, reflecting its compact structure that allows for efficient cellular translocation and interaction. Unlike nuclear-encoded peptides, MOTS-c's mitochondrial origin involves unique translation mechanics, where codons like AGA/AGG act as stop signals in mitochondria but code for arginine in cytoplasmic ribosomes, enabling its synthesis outside the organelle.
In lab settings, MOTS-c is typically supplied as a lyophilized powder with purity exceeding 98%, verified by HPLC and mass spectrometry. It demonstrates good solubility in aqueous buffers, and its levels in models decrease with age, suggesting relevance for age-related assays. Our transparency in testing ensures consistent quality, so you can confidently incorporate it into your protocols without variability concerns.
MOTS-c is a mitochondrial-derived peptide (MDP) consisting of 16 amino acids, encoded within the 12S rRNA region of the mitochondrial genome, and known for its role in regulating metabolic homeostasis and cellular stress responses in experimental models. This unique peptide, translated in the cytoplasm despite its mitochondrial origin, exhibits systemic effects that make it an intriguing subject for in-vitro studies exploring energy metabolism, aging processes, and inflammatory pathways. As with all our offerings, MOTS-c is provided exclusively for research purposes and in-vitro use only—not for human consumption or any clinical applications.
MOTS-c primarily functions through the folate-AICAR-AMPK pathway, where it inhibits the folate cycle and de novo purine biosynthesis, leading to AICAR accumulation and subsequent AMPK activation. This shifts cellular metabolism toward glucose utilization for pentose phosphate pathway activity, enhances NAD+ levels, and promotes mitochondrial biogenesis via PGC-1α upregulation. Under stress conditions like glucose restriction or oxidative challenge, MOTS-c translocates to the nucleus in an AMPK-dependent manner, interacting with transcription factors such as NRF2 to regulate antioxidant response elements (ARE) and mitigate ROS production.
Additionally, it exerts anti-inflammatory effects by reducing pro-inflammatory cytokines (e.g., TNF-α, IL-1β, IL-6) and boosting IL-10, potentially via NF-κB inhibition, while supporting insulin sensitivity through enhanced GLUT4 translocation. We're fond of how MOTS-c illustrates mito-nuclear communication—it's like a messenger optimizing energy balance under duress, and we're happy to guide you through assays that highlight these dynamics.
In essence, MOTS-c emerges as a fascinating mitochondrial-encoded peptide, offering researchers a window into metabolic regulation, stress adaptation, and age-related mechanisms through its AMPK-centered actions and systemic influences. Its potential to bridge mitochondrial and nuclear signaling excites our commitment to quality peptides that drive educational breakthroughs with integrity. Strictly for in-vitro use, MOTS-c captures our approachable spirit in supporting your scientific pursuits. Let's explore its depths together, one reasoned experiment at a time.
MOTS-c's metabolic regulatory profile makes it ideal for in-vitro explorations in obesity, diabetes, and aging models. In cell cultures from high-fat diet contexts, it has been used to study prevention of insulin resistance and obesity by enhancing energy expenditure and glucose clearance, with studies showing reduced body weight and improved skeletal muscle insulin sensitivity. Applications in gestational diabetes models demonstrate its ability to alleviate hyperglycemia and reduce offspring risks, offering insights into maternal-fetal metabolic interactions.
In aging research, MOTS-c supports investigations into physical decline, where it enhances endurance and reverses age-related insulin resistance in myocyte models, potentially extending lifespan by modulating methionine metabolism and NAD+ pathways. Inflammatory assays benefit from its cytokine-modulating effects, as seen in sepsis or lung injury models, where it reduces neutrophil infiltration and bacterial loads. Other areas include bone metabolism, with promotion of osteoblast proliferation and collagen production via TGF-β/SMAD signaling, and cardiovascular studies showing protection against endothelial dysfunction and calcification. We encourage using MOTS-c in stress-induction experiments to uncover its adaptive roles, and our educational tips can help refine your setups for optimal data.
To preserve MOTS-c's integrity in your research, store the lyophilized powder at -20°C or below in a dry, light-protected environment to prevent degradation. Reconstitute with sterile bacteriostatic water or a neutral buffer to concentrations of 1-2 mg/mL, gently swirling to dissolve without foaming, as this maintains its structural stability for assays.
Once prepared, aliquot solutions and use promptly, storing at 2-8°C for short-term or freezing for extended periods, while avoiding repeated freeze-thaw cycles. Our quality checks confirm its robustness, but always verify pH and clarity before use. If questions pop up, we're your go-to for logical handling advice.
MOTS-c is a mitochondrial-derived peptide (MDP) consisting of 16 amino acids, encoded within the 12S rRNA region of the mitochondrial genome, and known for its role in regulating metabolic homeostasis and cellular stress responses in experimental models. This unique peptide, translated in the cytoplasm despite its mitochondrial origin, exhibits systemic effects that make it an intriguing subject for in-vitro studies exploring energy metabolism, aging processes, and inflammatory pathways. As with all our offerings, MOTS-c is provided exclusively for research purposes and in-vitro use only—not for human consumption or any clinical applications.
MOTS-c primarily functions through the folate-AICAR-AMPK pathway, where it inhibits the folate cycle and de novo purine biosynthesis, leading to AICAR accumulation and subsequent AMPK activation. This shifts cellular metabolism toward glucose utilization for pentose phosphate pathway activity, enhances NAD+ levels, and promotes mitochondrial biogenesis via PGC-1α upregulation. Under stress conditions like glucose restriction or oxidative challenge, MOTS-c translocates to the nucleus in an AMPK-dependent manner, interacting with transcription factors such as NRF2 to regulate antioxidant response elements (ARE) and mitigate ROS production.
Additionally, it exerts anti-inflammatory effects by reducing pro-inflammatory cytokines (e.g., TNF-α, IL-1β, IL-6) and boosting IL-10, potentially via NF-κB inhibition, while supporting insulin sensitivity through enhanced GLUT4 translocation. We're fond of how MOTS-c illustrates mito-nuclear communication—it's like a messenger optimizing energy balance under duress, and we're happy to guide you through assays that highlight these dynamics.
In essence, MOTS-c emerges as a fascinating mitochondrial-encoded peptide, offering researchers a window into metabolic regulation, stress adaptation, and age-related mechanisms through its AMPK-centered actions and systemic influences. Its potential to bridge mitochondrial and nuclear signaling excites our commitment to quality peptides that drive educational breakthroughs with integrity. Strictly for in-vitro use, MOTS-c captures our approachable spirit in supporting your scientific pursuits. Let's explore its depths together, one reasoned experiment at a time.
MOTS-c features a 16-amino-acid sequence: MRWQEMGYIFYPRKLR, derived from a 51-base-pair short open reading frame (sORF) in the mitochondrial 12S rRNA gene (MT-RNR1), with high conservation across mammalian species, particularly in the first 11 residues. Its molecular weight is approximately 2,019 Da, reflecting its compact structure that allows for efficient cellular translocation and interaction. Unlike nuclear-encoded peptides, MOTS-c's mitochondrial origin involves unique translation mechanics, where codons like AGA/AGG act as stop signals in mitochondria but code for arginine in cytoplasmic ribosomes, enabling its synthesis outside the organelle.
In lab settings, MOTS-c is typically supplied as a lyophilized powder with purity exceeding 98%, verified by HPLC and mass spectrometry. It demonstrates good solubility in aqueous buffers, and its levels in models decrease with age, suggesting relevance for age-related assays. Our transparency in testing ensures consistent quality, so you can confidently incorporate it into your protocols without variability concerns.
MOTS-c's metabolic regulatory profile makes it ideal for in-vitro explorations in obesity, diabetes, and aging models. In cell cultures from high-fat diet contexts, it has been used to study prevention of insulin resistance and obesity by enhancing energy expenditure and glucose clearance, with studies showing reduced body weight and improved skeletal muscle insulin sensitivity. Applications in gestational diabetes models demonstrate its ability to alleviate hyperglycemia and reduce offspring risks, offering insights into maternal-fetal metabolic interactions.
In aging research, MOTS-c supports investigations into physical decline, where it enhances endurance and reverses age-related insulin resistance in myocyte models, potentially extending lifespan by modulating methionine metabolism and NAD+ pathways. Inflammatory assays benefit from its cytokine-modulating effects, as seen in sepsis or lung injury models, where it reduces neutrophil infiltration and bacterial loads. Other areas include bone metabolism, with promotion of osteoblast proliferation and collagen production via TGF-β/SMAD signaling, and cardiovascular studies showing protection against endothelial dysfunction and calcification. We encourage using MOTS-c in stress-induction experiments to uncover its adaptive roles, and our educational tips can help refine your setups for optimal data.
To preserve MOTS-c's integrity in your research, store the lyophilized powder at -20°C or below in a dry, light-protected environment to prevent degradation. Reconstitute with sterile bacteriostatic water or a neutral buffer to concentrations of 1-2 mg/mL, gently swirling to dissolve without foaming, as this maintains its structural stability for assays.
Once prepared, aliquot solutions and use promptly, storing at 2-8°C for short-term or freezing for extended periods, while avoiding repeated freeze-thaw cycles. Our quality checks confirm its robustness, but always verify pH and clarity before use. If questions pop up, we're your go-to for logical handling advice.
